女性无套免费网站在线看-女性特黄一级毛片-女神穿上情趣丝袜啪啪一整晚-女黄色片-成人在线午夜-成人在线小视频

咨詢熱線

15000266580

當前位置:首頁 >產品中心>細胞庫>人正常細胞>CRL-2854WPMY-1 人正常前列腺基質永生化細胞

WPMY-1 人正常前列腺基質永生化細胞

簡要描述:CRL-2854 WPMY-1 人正常前列腺基質永生化細胞,ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|;細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和培養條件!

  • 產品型號:CRL-2854
  • 廠商性質:生產廠家
  • 更新時間:2024-11-16
  • 訪  問  量:1678

產品分類

Product Category

詳細介紹

CRL-2854 WPMY-1 人正常前列腺基質永生化細胞

ATCC® Number:CRL-2854™    Price:
Designations:WPMY-1

Depositors:MM Webber

Biosafety Level:2 [Cells containing SV40 viral DNA sequence ]

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:myofibroblast


Source:Organ: prostate
Tissue: stroma
Disease: normal


Cellular Products:fibronectin [89997]

smooth muscle alpha-actin

vimentin [89997]



Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-2854 WPMY-1 人正常前列腺基質永生化細胞

Isolation:Isolation date: 1992

Receptors:androgen receptor, expressed ( [89997] upregulated upon exposure to androgen)

Tumorigenic:No

Antigen Expression:kallikrein 3, KLK3 (prostate specific antigen, PSA); Homo sapiens [89997]

Cytogenetic Analysis:At passage 66, a majority of the cells were in the 58-68 range; X, -Y. [89997]

Isoenzymes:AK-1, 1

ES-D, 2

G6PD, B

GLO-I, 1-2

Me-2, 0

PGM1, 2

PGM3, 1



Age:54 years

Gender:male

Ethnicity:Caucasian, White

Comments:The myofibroblast stromal cell line, WPMY-1, was derived from stromal cells from the same peripheral zone of the histologically normal adult prostate, as that used for RWPE-1 cells (ATCC CRL-11609). Stromal cells were immortalized with SV40-large-T antigen gene, using a pRSTV plasmid construct. WPMY-1 stromal cells belong to a family of cell lines derived from the same prostate as the epithelial RWPE-1 cells and all of its epithelial derivatives. Because of their derivation from the same peripheral zone of the prostate, the WPMY-1 cell line is especially useful for studies on paracrine and stromal : epithelial interactions.

The depositor reports that the RWPE-1 cell line (ATCC CRL-11609), derived from the same prostate, was screened for Hepatitis B and C, and human immunodeficiency viruses, and was found to be negative.



Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: Subculture cells before or upon reaching confluence. Do not allow cells to become super-confluent.


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS).

  3. Add 2.0 to 3.0 ml of 0.025% Trypsin - 0.26 mM EDTA solution (1:1 dilution of 0.05% Trypsin - 0.53 mM EDTA in D-PBS) to the flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of 0.1% Soybean Trypsin Inhibitor (or 2% fetal bovine serum in D-PBS) and aspirate cells by gently pipetting.

  5. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 x g for 5 to 10 minutes.

  6. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. An inoculum between 4 X 10(3) to 6 X 10(3) viable cells/sq. cm is recommended.

  7. Incubate cultures at 37C. We recommend that you maintain cultures at a cell concentraton between 6 X 10(3) and 5 X 10(4) cells/sq. cm.


Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 48 hours


Preservation:Freeze medium: Complete growth medium supplemented with an additional 15% fetal bovine serum and 10% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Doubling Time:38 hours

Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

derived from same individual:ATCC CRL-11609

derived from same individual:ATCC CRL-11610

derived from same individual:ATCC CRL-2849

derived from same individual:ATCC CRL-2850

derived from same individual:ATCC CRL-2852

derived from same individual:ATCC CRL-2851



References:

46793: Bello D, et al. Androgen responsive adult human prostatic epithelial cell lines immortalized by human papillomavirus 18. Carcinogenesis 18: 1215-1223, 1997. PubMed: 9214605
46795: Webber MM, et al. Acinar differentiation by non-malignant immortalized human prostatic epithelial cells and its loss by malignant cells. Carcinogenesis 18: 1225-1231, 1997. PubMed: 9214606
46799: Webber MM, et al. Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line. Carcinogenesis 17: 1641-1646, 1996. PubMed:8761420
46950: Okamoto M, et al. Interleukin-6 and epidermal growth factor promote anchorage-independent growth of immortalized human prostatic epithelial cells treated with N-methyl-N-nitrosourea. Prostate 35: 255-262, 1998. PubMed: 9609548
53180: Webber MM, et al. Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications. Part I. Cell markers and immortalized nontumorigenic cell lines. Prostate 29: 386-394, 1996. PubMed: 8977636
53181: Webber MM, et al. Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications Part 2. Tumorigenic cell lines. Prostate 30: 58-64, 1997. PubMed: 9018337
53182: Webber MM, et al. Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications. Part 3. Oncogenes, suppressor genes, and applications. Prostate 30: 136-142, 1997. PubMed: 9051152
53183: Kremer R, et al. ras Activation of human prostate epithelial cells induces overexpression of parathyroid hormone-related peptide. Clin. Cancer Res. 3: 855-859, 1997. PubMed: 9815759
53192: Jacob K, et al. Osteonectin promotes prostate cancer cell migration and invasion: a possible mechanism for metastasis to bone. Cancer Res. 59: 4453-4457, 1999. PubMed: 10485497
53193: Achanzar WE, et al. Cadmium induces c-myc, p53, and c-jun expression in normal human prostate epithelial cells as a prelude to apoptosis. Toxicol. Appl. Pharmacol. 164: 291-300, 2000. PubMed:10799339
53194: Achanzar WE, et al. Cadmium-induced malignant transformation of human prostate epithelial cells. Cancer Res. 61: 455-458, 2001. PubMed:11212230
53196: Bello-DeOcampo D, et al. Laminin-1 and alpha6beta1 integrin regulate acinar morphogenesis of normal and malignant human prostate epithelial cells. Prostate 46: 142-153, 2001. PubMed: 11170142
53197: Webber MM, et al. Human cell lines as an in vitro/in vivo model for prostate carcinogenesis and progression. Prostate 47: 1-13, 2001. PubMed: 11304724
89996: Quader ST, et al. Evaluation of the chemopreventive potential of retinoids using a novel in vitro human prostate carcinogenesis model. Mutat. Res. 496: 153-161, 2001. PubMed: 11551491
89997: Webber MM, et al. A human prostatic stromal myofibroblast cell line WPMY-1: a model for stromal-epithelial interactions in prostatic neoplasia. Carcinogenesis 20: 1185-1192, 1999. PubMed: 10383888
89998: Bello-DeOcampo D, et al. The role of alpha 6 beta 1 integrin and EGF in normal and malignant acinar morphogenesis of human prostatic epithelial cells. Mutat. Res. 480-481: 209-217, 2001. PubMed: 11506815
90372: Webber MM, et al. Modulation of the malignant phenotype of human prostate cancer cells by N-(4-hydroxyphenyl)retinamide (4-HPR). Clin. Exp. Metastasis 17: 255-263, 1999. PubMed: 10432011
90375: Sharp RM, et al. N-(4-hydroxyphenyl)retinamide (4-HPR) decreases neoplastic properties of human prostate cells: an agent for prevention. Mutat. Res. 496: 163-170, 2001. PubMed: 11551492
90376: Carruba G, et al. Regulation of cell-to-cell communication in non-tumorigenic and malignant human prostate epithelial cells. Prostate 50: 73-82, 2002. PubMed: 11816015

CRL-2854




















產品咨詢

留言框

  • 產品:

  • 您的單位:

  • 您的姓名:

  • 聯系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結果(填寫阿拉伯數字),如:三加四=7
聯系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術支持:化工儀器網    管理登陸
主站蜘蛛池模板: 亚洲精品视频久久久 | 亚洲一区二区三区高清 | 怡红院免费的全部视频国产a | 免费aⅴ片| 国产男女视频 | 欧美一线不卡在线播放 | 手机在线播放视频 | 99视频精品全国免费 | 五月色婷婷综合开心网4438 | 国产精品美女一区二区 | 国产精品短视频免费观看 | 欧美成人在线免费 | 日韩欧美第一页 | 真人一级毛片免费观看视频 | 欧美成人26uuu欧美毛片 | 亚洲视屏在线观看 | 国产欧美日韩不卡一区二区三区 | 九九精品视频在线观看九九 | 成人在免费观看视频国产 | 亚洲欧美日韩综合久久久久 | 国产精选莉莉私人影院 | 久久精品中文字幕不卡一二区 | 欧美一区二区三区视视频 | 亚洲一区二区三区久久 | 久久国产精品久久精 | 精品九九视频 | 亚洲人成在线影院 | 99精品视频在线观看免费 | 亚洲精品成人一区二区 | 欧美一级视频高清片 | 波多野结衣在线视频观看 | 国产成人深夜福利在线观看 | 欧美精品自拍 | 另类视频欧美 | 正在播放亚洲一区 | 香蕉依依精品视频在线播放 | 国产成人久久精品激情91 | 日韩精品久久一区二区三区 | 亚洲国产日韩综合久久精品 | 久久全国免费久久青青小草 | 国产高清在线视频 |