女性无套免费网站在线看-女性特黄一级毛片-女神穿上情趣丝袜啪啪一整晚-女黄色片-成人在线午夜-成人在线小视频

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細胞庫>人正常細胞>CRL-2888WPE-int 人正常前列腺上皮細胞

WPE-int 人正常前列腺上皮細胞

簡要描述:CRL-2888 WPE-int 人正常前列腺上皮細胞,原代細胞|細胞系|細胞株|菌種原代細胞、細胞系。細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和*培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-2888
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-16
  • 訪  問  量:1441

產(chǎn)品分類

Product Category

詳細介紹

CRL-2888 WPE-int 人正常前列腺上皮細胞

ATCC® Number:CRL-2888™    Price:$329.00
Designations:WPE-int

Depositors:MM Webber, EJ Tokar

Biosafety Level:2 [cells contain human HPV-18 viral DNA sequences ]

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:epithelial
WPE-int 人正常前列腺上皮細胞


Source:Organ: prostate, normal, peripheral zone
Cell Type: epithelial


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-2888 WPE-int 人正常前列腺上皮細胞

Isolation:Isolation date: 2004 [PubMed: 16351690] [92794]

Receptors:androgen receptor, expressed ( [92794] upregulated upon exposure to androgen)

Tumorigenic:NO

Antigen Expression:kallikrein 3 ( KLK3); prostate specific antigen (PSA); expressed (upregulated upon exposure to androgen) [PubMed: 16351690] [92794]

DNA Profile (STR):Amelogenin: X,Y

CSF1PO: 13

D13S317: 8,14

D16S539: 9,11

D5S818: 12,15

D7S820: 10,11

THO1: 8

TPOX: 8,11

vWA: 14,18



Age:54

Gender:male

Ethnicity:Caucasian

Comments:WPE-int cells were derived from the RWPE-1 cell line (ATCC CRL-11609) after two consecutive cycles of single cell cloning. To establish the RWPE-1 cell line, epithelial cells from the peripheral zone of a histologically normal adult human prostate were transfected with a plasmid carrying one copy of the human papilloma virus 18 (HPV-18) genome. WPE-int cells exhibit features characteristic of an intermediate cell type on the path to luminal cell differentiation. Cells show very low expression of p63 and MMP-2 and high expression of cytokeratin 18 as compared to the WPE-stem cell line (ATCC CRL-2887). They are anchorage-dependent. [PubMed: 16351690]The WPE-stem cell line (ATCC CRL-2887), which expresses several features characteristic of stem/progenitor cells, was also derived from RWPE-1 cells after single cell cloning.
  1. The parental cell line RWPE-1 was screened for CMV, HBV, HCV, HTLV 1, HTLV 2, HIV 1, HIV 2, JCV, and MoMuLV DNA sequences. Cells were also tested for 25 species of mycoplasma and Acholeplasma laidlawii . Cells tested negative for all of the above. (personal communication from depositor).



Propagation:ATCC complete growth medium: The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium:
  • 0.05 mg/ml BPE - provided with the K-SFM kit

  • 5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium.
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37.0°C
    Growth Conditions: Subculture cells before they reach confluence. Do not allow cells to become confluent.




CRL-2888

ATCC® Number: CRL-2888™ Price: $329.00
Designations: WPE-int
Depositors: MM Webber, EJ Tokar
Biosafety Level: 2 [cells contain human HPV-18 viral DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial


Source: Organ: prostate, normal, peripheral zone
Cell Type: epithelial
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: 2004 [PubMed: 16351690] [92794]
Receptors: androgen receptor, expressed ( [92794] upregulated upon exposure to androgen)
Tumorigenic: NO
Antigen Expression: kallikrein 3 ( KLK3); prostate specific antigen (PSA); expressed (upregulated upon exposure to androgen) [PubMed: 16351690] [92794]
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 13
D13S317: 8,14
D16S539: 9,11
D5S818: 12,15
D7S820: 10,11
THO1: 8
TPOX: 8,11
vWA: 14,18
Age: 54
Gender: male
Ethnicity: Caucasian
Comments: WPE-int cells were derived from the RWPE-1 cell line (ATCC CRL-11609) after two consecutive cycles of single cell cloning. To establish the RWPE-1 cell line, epithelial cells from the peripheral zone of a histologically normal adult human prostate were transfected with a plasmid carrying one copy of the human papilloma virus 18 (HPV-18) genome. WPE-int cells exhibit features characteristic of an intermediate cell type on the path to luminal cell differentiation. Cells show very low expression of p63 and MMP-2 and high expression of cytokeratin 18 as compared to the WPE-stem cell line (ATCC CRL-2887). They are anchorage-dependent. [PubMed: 16351690]The WPE-stem cell line (ATCC CRL-2887), which expresses several features characteristic of stem/progenitor cells, was also derived from RWPE-1 cells after single cell cloning.
The parental cell line RWPE-1 was screened for CMV, HBV, HCV, HTLV 1, HTLV 2, HIV 1, HIV 2, JCV, and MoMuLV DNA sequences. Cells were also tested for 25 species of mycoplasma and Acholeplasma laidlawii . Cells tested negative for all of the above. (personal communication from depositor).
Propagation: ATCC complete growth medium: The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium:
•0.05 mg/ml BPE - provided with the K-SFM kit
•5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: Subculture cells before they reach confluence. Do not allow cells to become confluent.



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
主站蜘蛛池模板: 美女黄色免费看 | 亚洲成人手机在线 | 久久国产精品高清一区二区三区 | 真实国产普通话对白乱子子伦视频 | 久久天天躁综合夜夜黑人鲁色 | 欧美日韩99 | 日本一级毛片视频无遮挡免费 | 国产一级网站 | 国产在线91精品天天更新 | 精品在线视频免费观看 | 国产一级久久免费特黄 | 国产丝袜不卡一区二区 | 五月色婷婷琪琪综合伊人 | 亚洲国产精品aaa一区 | 日韩福利视频精品专区 | 国产精品久久久久久久 | 欧美另类视频在线 | 午夜一级毛片不卡 | 中日韩精品视频在线观看 | 在线はじめてのおるすばん | 国产在线精品福利91香蕉 | 日本综合欧美一区二区三区 | 国产精品资源手机在线播放 | 欧美在线一级毛片视频 | 亚洲第3页| 亚洲精品视频在线 | 夜间福利在线观看 | 亚洲女人网 | 免费一级欧美毛片 | 亚洲欧美在线免费 | 一区视频在线 | 欧美国产精品不卡在线观看 | 中文字幕有码在线 | 欧美日韩视频在线第一区二区三区 | 日韩午夜视频在线观看 | 男女视频在线观看 | 98国内自拍在线视频 | 中文字幕亚洲高清综合 | 精品国产亚洲一区二区三区 | 97成人在线视频 | 一区二区在线播放福利视频 |