女性无套免费网站在线看-女性特黄一级毛片-女神穿上情趣丝袜啪啪一整晚-女黄色片-成人在线午夜-成人在线小视频

咨詢(xún)熱線(xiàn)

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CCL-138Detroit 562細(xì)胞, 人咽頭癌胸水轉(zhuǎn)移細(xì)胞

Detroit 562細(xì)胞, 人咽頭癌胸水轉(zhuǎn)移細(xì)胞

簡(jiǎn)要描述:Detroit 562細(xì)胞, 人咽頭癌胸水轉(zhuǎn)移細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

  • 產(chǎn)品型號(hào):CCL-138
  • 廠(chǎng)商性質(zhì):生產(chǎn)廠(chǎng)家
  • 更新時(shí)間:2024-11-16
  • 訪(fǎng)  問(wèn)  量:1605

產(chǎn)品分類(lèi)

Product Category

詳細(xì)介紹

Detroit 562細(xì)胞, 人咽頭癌胸水轉(zhuǎn)移細(xì)胞

OrganismHomo sapiens, human
Tissuepharynx: derived from metastatic site: pleural effusion
Product Formatfrozen
Morphologyepithelial
Culture Propertiesadherent
Biosafety Level1
Diseasepharyngeal carcinoma
Age*****
Genderfemale
EthnicityCaucasian
Storage Conditionsliquid nitrogen vapor phase

 Karyotype   Modal number = 64; range = 58 to128
A large subterminal marker chromosome, arm ratio 3:4, is found in 94% of the cells karyotyped. Five to 6 minute chromosomes are present in each cell. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines   Clinical Data  

Detroit 562細(xì)胞, 人咽頭癌胸水轉(zhuǎn)移細(xì)胞

Caucasian

female

 Genes Expressed  

keratin

 Virus Susceptibility   Human poliovirus 1
Vesicular stomatitis virus              
 Comments  

The cells are positive for keratin by immunoperoxidase staining.

Complete Growth MediumThe base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing





Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Cryopreservation

Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage Temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: Air, 95%; carbon dioxide (CO2), 5%

Temperature: 37°C              CCL-138 Detroit 562 人咽頭癌胸水轉(zhuǎn)移細(xì)胞























產(chǎn)品咨詢(xún)

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話(huà):

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢(xún)熱線(xiàn)

400-821-8510

(周一至周日9:00- 19:00)

在線(xiàn)咨詢(xún)
  • 掃一掃 微信咨詢(xún)

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
主站蜘蛛池模板: 亚洲高清成人欧美动作片 | 亚洲欧洲一区二区三区在线 | 怡红院在线观看在线视频 | 91久久综合| 美国毛片一级视频在线aa | 欧美日韩在线视频一区 | 国产精品网址 | 瑟瑟网站在线观看 | 国产亚洲精品一区久久 | 久久精品中文字幕首页 | 亚洲国产成人在线视频 | 日本一区二区三区四区五区 | 日本一区二区三区四区无限 | 成人午夜视频在线播放 | 久久国产精品99久久久久久牛牛 | 国产呦精品一区二区三区网站 | 男女性高爱潮免费的国产 | 欧美 在线 成 人怡红院 | 亚洲免费视频网 | 亚洲精品手机在线 | 免费观看亚洲视频 | 欧美日韩视频精品一区二区 | 国内精品久久久久影院亚洲 | 久久成人免费观看草草影院 | 97se亚洲综合在线韩国专区福利 | 久久久久亚洲精品影视 | 色三级大全高清视频在线观看 | 亚洲欧美一二三区 | 国产丶欧美丶日韩丶不卡影视 | 在线精品播放 | 大陆精品自在线拍国语 | 国产一区二区三区亚洲欧美 | 99久久精品免费看国产一区二区 | 欧美特级午夜一区二区三区 | 国产日韩欧美自拍 | 91久久精品国产91久久性色tv | 欧美成人 一区二区三区 | 91精品视频免费 | 久久香蕉国产线看免费 | 中文字幕 亚洲精品 第1页 | 国产一区亚洲欧美成人 |