女性无套免费网站在线看-女性特黄一级毛片-女神穿上情趣丝袜啪啪一整晚-女黄色片-成人在线午夜-成人在线小视频

咨詢(xún)熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>CRL-2741HBE135-E6E7人支氣管上皮細(xì)胞

HBE135-E6E7人支氣管上皮細(xì)胞

簡(jiǎn)要描述:HBE135-E6E7人支氣管上皮細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-2741
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-21
  • 訪  問(wèn)  量:3195

產(chǎn)品分類(lèi)

Product Category

詳細(xì)介紹

HBE135-E6E7人支氣管上皮細(xì)胞

細(xì)胞貨期8-10個(gè)工作日

上海復(fù)祥生物提供 ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件,上有細(xì)胞照片,歡迎各位老師!xiangfbio.


說(shuō)明書(shū):Volumes used in this protocol are for 75 cm2 flask;             proportionally reduce or increase amount of dissociation medium         for culture vessels of other sizes.


HBE135-E6E7人支氣管上皮細(xì)胞

細(xì)胞貨期8-10個(gè)工作日

上海復(fù)祥生物提供 ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件,上有細(xì)胞照片,歡迎各位老師!xiangfbio.


說(shuō)明書(shū):Volumes used in this protocol are for 75 cm2 flask;             proportionally reduce or increase amount of dissociation medium         for culture vessels of other sizes.



細(xì)胞貨期8-10個(gè)工作日

上海復(fù)祥生物提供 ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件,上有細(xì)胞照片,歡迎各位老師!xiangfbio.


說(shuō)明書(shū):Volumes used in this protocol are for 75 cm2 flask;             proportionally reduce or increase amount of dissociation medium         for culture vessels of other sizes.


Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells     under an inverted microscope until       cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping, do not agitate the cells     by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by pipetting gently.

To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 x g for 5 to 10 minutes.

Discard supernatant and resuspend cells in fresh serum-free growth medium.  Add appropriate aliquots of cell suspension to new culture vessels.

Place culture vessels in incubators at 37°C.


Subc*tion Ratio: 1:3 to 1:4

Medium Renewal: Every 2 to 3 days.

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells: a Manual of Basic Technique by R. Ian Freshney, 3th edition, published by Alan R. Liss, N.Y., 1994.

培養(yǎng)條件:Keratinocyte-Serum Free medium with 5 ng/ml human recombinant EGF (do not filter) and 0.05 mg/ml bovine pituitary extract (Invitrogen, formerly GIBCO-BRL, Cat.  17005-042) and supplemented with 0.005 mg/ml insulin and 500 ng/ml hydrocortisone.
























產(chǎn)品咨詢(xún)

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢(xún)熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢(xún)
  • 掃一掃 微信咨詢(xún)

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
主站蜘蛛池模板: 91久久精品一区二区 | 日本成人免费在线 | 女人张开腿 让男人桶视频 女人张开腿等男人桶免费视频 | 色丁香久久| 美女视频全部免费 | 成人a毛片免费视频观看 | 99久久精品国产一区二区三区 | 精品乱人伦一区二区 | 久久久青草 | 一区二区三区四区在线视频 | 一级片免费视频 | 久久久久久一级毛片免费无遮挡 | 欧美黄视频在线观看 | 国产不卡影院 | 一色屋精品亚洲香蕉网站 | 久久综合久久精品 | 精品亚洲视频在线 | 欧美一级特黄特色大片免费 | 免费又黄又爽的视频 | 国产精品揄拍一区二区久久 | 国产成人精品男人的天堂538 | 黄色成人在线 | 欧美特级视频 | 青青影院一区二区免费视频 | 欧美成人免费夜夜黄啪啪 | 欧美孕交视频 | 亚洲国产欧美日韩精品一区二区三区 | 欧美视频自拍偷拍 | 加勒比综合网 | 男人的天堂久久爱 | 亚洲欧美日韩精品香蕉 | 久久精品国产99精品最新 | 久久久久久一级毛片免费野外 | 一本一道波多野结衣456 | 成人午夜私人影院入口 | 国产成人精品男人的天堂538 | 国产欧美日韩在线观看一区二区三区 | 精品视频一二三区 | 免费三级网站 | 亚洲精品精品 | 日本午夜vr影院新入口 |