咨詢熱線
15000266580
15000266580
當前位置：首頁 >產(chǎn)品中心>細胞庫>人腫瘤細胞、癌細胞>BC-1人lymphoma B 淋巴細胞
簡要描述：CRL-2230 BC-1 人lymphoma B 淋巴細胞，ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|，細胞庫管理規(guī)范，提供的細胞株背景清楚，提供參考文獻和培養(yǎng)條件！
產(chǎn)品型號：BC-1廠商性質(zhì)：生產(chǎn)廠家 更新時間：2024-11-12訪 問 量：2013 產(chǎn)品分類
Product Category相關(guān)文章
Related Articles詳細介紹
CRL-2230 BC-1 人lymphoma B 淋巴細胞
ATCrC® Numbe: CRL-2230™ Price: $417.00
Designations: BC-1
Depositoo Level: 2 [CELLS CONTAIN HERPESVIRUS ]
Shipped: frzen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens （human）
Morphology: lymphoblast
CRL-2230 BC-1 人lymphomars: E Cesarman
Biosafety
B 淋巴細胞
Source: Disease: lymphoma
Cell Type: B lymphocyte;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host （Roche FuGENE® Transfection Reagents）
Antigen Expression: CD30 +; CD45 +; EMA + （epithelial membrane antigen）
DNA Profile （STR）: Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 8
D16S539: 12,13
D5S818: 11,12
D7S820: 11
THO1: 9.3
TPOX: 8,9
vWA: 16,20
Age: 46 years
Gender: male
Comments: BC-1 is a lymphoma cell line originated in 1992 by Ethel Cesarman and Giorgio Inghirami in the laboratory of Daniel M. Knowles.
The cells were derived from an acquired immunodeficiency syndrome （AIDS） related body cavity based lymphoma presenting as a primary lymphomatous effusion in the peritoneal cavity.
The cells contain two viral genomes: Epstein-Barr virus （EBV） and Kaposi sarcoma associated herpesvirus （KSHV, provisionally designed HHV-8）.
KSHV is a recently identified and largely uncharacterized virus.
The BC-1 cell line allows in vitro culture of KSHV viral genomes.
It is also a practical source of KSHV DNA to be used as a positive control for screening tests.
Propagation: ATCC complete growth medium: RPMI 1640 medium, 80%; fetal bovine serum, 20%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp5 cells/ml and maintain between 5 X 10 exp5 and 2 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
Doubling Time: 48 to 72 hrs
References: 22572: Moore PS, et al. Primary characterization of a herpesvirus agent associated with Kaposi's sarcoma. J. Virol. 70: 549-558, 1996. PubMed: 8523568
23358: Cesarman E, et al. In vitro establishment and characterization of two acquired immunodeficiency syndrome-related lymphoma cell lines （BC-1 and BC-2） containing Kaposi's sarcoma-associated herpesvirus-like （KSHV） DNA sequences. Blood 86: 2708-2714, 1995. PubMed: 7670109
23395: Chadburn A, et al. CD30 （Ki-1） positive anaplastic large cell lymphomas in individuals infected with the human immunodeficiency virus. Cancer 72: 3078-3090, 1993. PubMed: 8221575
23493: Cesarman E, et al. Kaposi's sarcoma-associated herpesvirus-like DNA sequences in AIDS- related body-cavity-based lymphomas. N. Engl. J. Med. 332: 1186-1191, 1995. PubMed: 7700311
CRL-2230 BC-1 人lymphoma B 淋巴細胞
產(chǎn)品咨詢
郵箱：xiangfbio@163.com
地址：上海市虹口區(qū)四平路710號7層
掃一掃 微信咨詢