女性无套免费网站在线看-女性特黄一级毛片-女神穿上情趣丝袜啪啪一整晚-女黄色片-成人在线午夜-成人在线小视频

咨詢熱線

15000266580

當前位置:首頁 >產品中心>細胞庫>大鼠正常細胞>CRL-1589 IEC-18 大鼠回腸細胞

CRL-1589 IEC-18 大鼠回腸細胞

簡要描述:CRL-1589 IEC-18 大鼠回腸細胞, ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和培養條件

  • 產品型號:
  • 廠商性質:生產廠家
  • 更新時間:2024-11-14
  • 訪  問  量:2037

產品分類

Product Category

詳細介紹

CRL-1589 IEC-18 大鼠回腸細胞, ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻i優培養條件

CRL-1589 IEC-18 大鼠回腸細胞 的詳細介紹


ATCC® Number:CRL-1589™  Price:$329.00
Designations:IEC-18

Depositors:A Quaroni

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Rattus norvegicus (rat)

Morphology:epithelial


Source:Organ: ileum
Cell Type: epithelial


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Propagation:ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 Unit/ml bovine insulin, 95%; fetal bovine serum, 5%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37C.


Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended
Medium Renewal: Twice per week


Preservation:Freeze medium: culture medium 82.5%; fetal bovine serum 10%; DMSO 7.5%
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101

Cell culture tested DMSO:ATCC 4-X



References:22491: Quaroni A, et al. Epithelioid cell cultures from rat small intestine. Characterization by morphologic and immunologic criteria. J. Cell Biol. 80: 248-265, 1979. PubMed: 88453

26096: Quaroni A, Isselbacher KJ. Cytotoxic effects and metabolism of benzo[a]pyrene and 7,12- dimethylbenz[a]anthracene in duodenal and ileal epithelial cell cultures. J. Natl. Cancer Inst. 67: 1353-1362, 1981. PubMed: 6273638





















產品咨詢

留言框

  • 產品:

  • 您的單位:

  • 您的姓名:

  • 聯系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結果(填寫阿拉伯數字),如:三加四=7
聯系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術支持:化工儀器網    管理登陸
主站蜘蛛池模板: 在线观看免费视频国产 | 精品欧美成人bd高清在线观看 | 成人性视频在线三级 | 欧美亚洲不卡 | 毛片视频免费观看 | 亚洲国产精品久久综合 | 国产99视频精品免费视频免里 | 亚洲精品美女视频 | 免费 视频 1级 | 日韩欧美亚洲视频 | 91.久久 | 99久久免费国产精精品 | 欧美亚洲黄色 | 精品欧美成人高清视频在线观看 | 国内精品久久久久久久久蜜桃 | 一级特级欧美aaaaa毛片 | 日韩亚洲欧美一区 | 特级毛片在线播放 | 免费萌白酱国产一区二区三区 | 美女视频黄的免费看网站 | 亚洲成a人片毛片在线 | 免费男女视频 | 男女性男女刺激大片免费观看 | 99视频久久精品久久 | 91国内视频 | 日本一区二区三区不卡在线视频 | 欧美做爱毛片 | 久久se精品一区二区国产 | 亚洲天堂美女视频 | 韩国三级日本三级香港三级黄 | 亚洲视频免费在线观看 | 亚洲综合国产一区二区三区 | 日韩偷拍自拍 | 加勒比日本道 | 国产精品a人片在线观看 | 男人天堂avav | 99久久国产免费 - 99久久国产免费 | 萌白酱粉嫩jk福利在线观看 | 亚洲国产小视频 | 欧美一级欧美一级在线播放 | 一区二区三区免费视频播放器 |