女性无套免费网站在线看-女性特黄一级毛片-女神穿上情趣丝袜啪啪一整晚-女黄色片-成人在线午夜-成人在线小视频

咨詢熱線

15000266580

當前位置:首頁 >產品中心>細胞庫>大鼠正常細胞>CRL-1442 BRL 3A 大鼠肝細胞

CRL-1442 BRL 3A 大鼠肝細胞

簡要描述:CRL-1442 BRL 3A 大鼠肝細胞, ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和培養條件

  • 產品型號:
  • 廠商性質:生產廠家
  • 更新時間:2024-11-14
  • 訪  問  量:2102

產品分類

Product Category

詳細介紹

CRL-1442 BRL 3A 大鼠肝細胞, ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻i優培養條件


CRL-1442 BRL 3A 大鼠肝細胞 的詳細介紹


ATCC® Number:  CRL-1442™

Designations:  BRL 3A

Depositors:   SP Nissley, MM Rechler

Biosafety Level: 1

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Rattus norvegicus (rat)

Morphology:


Source: Organ: liver

Strain: Buffalo

Cellular Products: somatomedin like multiplication stimulating activity (MSA)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Comments: The serum-free conditioned medium from this cell line is a source of MSA. MSA is a family of polypeptides that can partially satisfy the serum reguirement of chick embryo fibroblasts. [1060]

Propagation:  ATCC complete growth medium: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Growth Conditions: For 24 hrs after initiating culture from a thawed ampule or after subculturing, grow the cells in culture medium with 5% fetal bovine serum. After 24 hrs, the cells may be transferred to serum-free medium.

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 xg for 5 to 10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.

Incubate cultures at 37°C.




Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended

Medium Renewal: 2 times per week

Preservation:  Freeze medium: Complete growth medium supplemented with 10% fetal bovine serum and 5% DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

References: 1060: Nissley SP, et al. Proliferation of buffalo rat liver cells in serum-free medium does not depend upon multiplication-stimulating activity (MSA). Cell 11: 441-446, 1977. PubMed: 302146

22582: Coon HG, Weiss MC. A quantitative comparison of formation of spontaneous and virus- produced viable hybrids. Proc. Natl. Acad. Sci. USA 62: 852-859, 1969. PubMed: 4308097

22711: Dulak NC, Temin HM. A partially purified polypeptide fraction from rat liver cell conditioned medium with multiplication-stimulating activity for embryo fibroblasts. J. Cell. Physiol. 81: 153-170, 1973. PubMed: 4735141

22712: Dulak NC, Shing YW. Large scale purification and further characterization of a rat liver cell conditioned medium multiplication stimulating activity. J. Cell. Physiol. 90: 127-130, 1977. PubMed: 833209

22762: Schalch DS, et al. Nonsuppressible insulin-like activity (NSILA). I. Development of a new sensitive competitive protein-binding assay for determination of serum levels. J. Clin. Endocrinol. Metab. 46: 664-671, 1978. PubMed: 755052























產品咨詢

留言框

  • 產品:

  • 您的單位:

  • 您的姓名:

  • 聯系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結果(填寫阿拉伯數字),如:三加四=7
聯系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術支持:化工儀器網    管理登陸
主站蜘蛛池模板: 精品国产91在线网 | 九九国产精品 | 天天澡天天碰天天狠伊人五月 | 老司机午夜性生免费福利 | 久久久久久久久久久福利观看 | 国产成人久久一区二区三区 | 美国毛片亚洲社区在线观看 | 九九视频免费精品视频免费 | 可以免费看黄色的网站 | 日韩欧美在线观看视频一区二区 | 欧美一级毛片生活片 | 国产精品精品国产一区二区 | 国内国产真实露脸对白 | 久久99国产亚洲精品 | 亚洲国产一区二区三区四区五区 | 欧美日韩国产在线人成dvd | 国产成人看片免费视频观看 | 日本私人色多多 | 国产欧美日韩视频在线观看一区二区 | 亚洲欧洲无码一区二区三区 | 中文一区 | 美女被男人桶到嗷嗷叫爽网站 | 亚洲精品综合 | 亚洲一区二区三区高清视频 | 精品国产高清久久久久久小说 | 久久久久久久久久久9精品视频 | 草草影院ccyy国产日本欧美 | 国产精品久久久久久亚洲伦理 | 欧美在线视频观看 | 男女视频在线观看免费高清观看 | 久久精品国产欧美日韩99热 | 国产午夜爽爽窝窝在线观看 | 日韩亚洲一区中文字幕在线 | 国产乱子精品免费视观看片 | 国产一区二区三区日韩 | 国产一级片毛片 | 亚洲图片视频在线 | 国产精品亚洲一区二区三区在线观看 | 亚洲国产二区三区久久 | 久久er国产精品免费观看1 | 亚洲无色|