女性无套免费网站在线看-女性特黄一级毛片-女神穿上情趣丝袜啪啪一整晚-女黄色片-成人在线午夜-成人在线小视频

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>大鼠正常細(xì)胞>HIT-T15 大鼠胰腺B細(xì)胞

HIT-T15 大鼠胰腺B細(xì)胞

簡要描述:HIT-T15 大鼠胰腺B細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問  量:1999

產(chǎn)品分類

Product Category

詳細(xì)介紹

HIT-T15 大鼠胰腺B細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)i優(yōu)培養(yǎng)條件


HIT-T15 大鼠胰腺B細(xì)胞

ATCC® Number: CRL-1777™    Price: $289.00
Designations: HIT-T15

Depositors: R Santerre

Biosafety Level:2 [Cells Contain PAPOVAVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties:adherent

Organism:Mesocricetus auratus (hamster, Syrian golden)

Morphology:epithelial
HIT-T15 大鼠胰腺B細(xì)胞


Source:Organ: pancreas
Tissue: islet of Langerhans
Cell Type: beta cell;


Cellular Products:insulin

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Applications:transfection host (Roche FuGENE® Transfection Reagents)

Receptors:glucagon; somatostatin; glucocorticoid

Comments:This line was established from a primary culture of Syrian hamster islet cells which were transformed with SV40.

The cells secrete up to 24 ng of insulin per 10 exp6 cells per 24 hours (passage 60).

Insulin secretion is stimulated by glucose and glucagon, and is suppressed by somatostatin and glucocorticoids.

Insulin synthesis decreases with length of time in culture.



Propagation: ATCC complete growth medium: Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 87.5%; dialyzed horse serum, 10%; fetal bovine serum, 2.5%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C


Subculturing: Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.


  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.























產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
主站蜘蛛池模板: 欧美成人另类 | 99精品视频在线观看re | 亚洲精品大片 | 美女在线网站免费的 | 久久丁香 | 亚洲你我色 | 欧洲成人r片在线观看 | 波多野结衣视频免费在线观看 | 日本免费成人网 | 欧美午夜网站 | 在线观看国产欧美 | 国产成人精品久久综合 | 国产婷婷一区二区在线观看 | 久国产 | 亚洲视频一区在线 | 一级国产交换配乱淫 | 又黄又免费的网站 | 国产一区二区影视 | 欧美在线观看www | 免费播放欧美毛片欧美aaaaa | 偷自拍| 国产精品欧美激情在线播放 | 国产精品一国产精品免费 | 91高清国产经典在线观看 | 久久久高清免费视频 | 色偷偷女男人的天堂亚洲网 | 高清国产一区二区三区 | 91精品91 | 日本噜噜影院 | 男人的天堂在线观看免费 | 亚洲国产激情一区二区三区 | 韩国在线精品福利视频在线观看 | 久热免费在线观看 | 中文字幕日韩精品有码视频 | 日本免费毛片在线高清看 | 欧美特黄一级aa毛片 | 国产一级特黄特色aa毛片 | 国产色a| 视频偷拍一级视频在线观看 | 国产成人精品视频频 | 久久高清一区二区三区 |