女性无套免费网站在线看-女性特黄一级毛片-女神穿上情趣丝袜啪啪一整晚-女黄色片-成人在线午夜-成人在线小视频

咨詢熱線

15000266580

當前位置:首頁 >產品中心>細胞庫>人腫瘤細胞、癌細胞>CRL-1690 T98G 人多形性惡性膠質瘤細胞

CRL-1690 T98G 人多形性惡性膠質瘤細胞

簡要描述:CRL-1690 T98G 人多形性惡性膠質瘤細胞, ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|。細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和培養條件!

  • 產品型號:
  • 廠商性質:生產廠家
  • 更新時間:2024-11-14
  • 訪  問  量:2807

產品分類

Product Category

詳細介紹

CRL-1690 T98G  人多形性惡性膠質瘤細胞

ATCC® Number:CRL-1690™Price:$279.00
Designations:T98G [T98-G]

Depositors:GH Stein

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:fibroblast


Source:Organ: brain

Disease: glioblastoma multiforme



Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Applications:transfection host

Tumorigenic:CRL-1690 T98G  人多形性惡性膠質瘤細胞

DNA Profile (STR):Amelogenin: X,Y

CSF1PO: 10,12

D13S317: 13

D16S539: 13

D5S818: 10,12

D7S820: 9,10

THO1: 7,9.3

TPOX: 8

vWA: 17,20



Cytogenetic Analysis:This is a human cell line with hyperpentaploid chromosome count. The modal chromosome number should be around 128 to 132. The rate of cells with higher ploidies was 1.39%. Fourteen to 16 marker chromosomes were common to most cells. They were: der(1)t(1;?) (p36;?), i(6p), der(10)t(10;?) (q24;?), der (19)t(19;?) (q13;?), der(15)t(15;?) (q26?;?), minute metacentric and eight to ten others. Most of these structurally altered markers had complex interchromosomal exchanges. The der(10) and der(19) could be formed from a balanced translocation, i.e., t(10;19) (q24;q13). These two markers and the minute metacentric were present in three or more copies in most cells. There were six or more copies for N5, N7, N11, N13, N20, N21, and N22 in most cells. The X and N15 had only one copy.

Age:61 years  CRL-1690 T98G  人多形性惡性膠質瘤細胞

Gender:male

Ethnicity:Caucasian

Comments:When deprived of serum or when crowded, the cells enter a viable G1 arrested state.

The cells are anchorage independent.



Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C



Subculturing:

Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

CRL-1690 T98G  人多形性惡性膠質瘤細胞

  • Remove and discard culture medium.

  • Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  • Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

  • Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting

  • Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.

  • Incubate cultures at 37C.

CRL-1690 T98G  人多形性惡性膠質瘤細胞

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:10 is recommended

Medium Renewal: 2 to 3 times per week



Preservation:Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase



Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020



References:22322: Stein GH. T98G: an anchorage-independent human tumor cell line that exhibits stationary phase G1 arrest in vitro. J. Cell. Physiol. 99: 43-54, 1979. PubMed: 222778

23094: Olopade OI, et al. Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res. 52: 2523-2529, 1992. PubMed: 1568221

32287: Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024


CRL-1690 T98G  人多形性惡性膠質瘤細胞




















產品咨詢

留言框

  • 產品:

  • 您的單位:

  • 您的姓名:

  • 聯系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結果(填寫阿拉伯數字),如:三加四=7
聯系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術支持:化工儀器網    管理登陸
主站蜘蛛池模板: 成人一a毛片免费视频 | 国产美女做爰免费视频网址 | 艳女伦交一级毛片 | 美女一级毛片毛片在线播放 | 亚洲国产午夜精品理论片的软件 | 免费国产成人 | 免费视频18 | 在线亚洲欧美日韩 | 男人操美女网站 | 一级特黄特色的免费大片视频 | 日本高清视频www夜色资源 | 欧美精品久久久久久久影视 | 一男一女的一级毛片 | 久久久999国产精品 久久久99精品免费观看 | 成人在线毛片 | 手机看片免费基地你懂的 | 国产日韩欧美自拍 | 亚洲综合一 | 亚洲乱人伦精品图片 | 91视频啪啪 | 欧美性一区二区三区 | 免费一看一级欧美 | 中文字幕在线观看一区二区 | 爱爱爱久久久久久久 | 国产毛片一区二区三区精品 | 黄色一级毛片免费 | 国产在线精品一区二区三区 | 国产欧美另类久久久精品免费 | 欧美另类交视频 | 久久久久久久国产 | 韩日一级| 怡红院亚洲红怡院天堂麻豆 | 日本高清视频免费在线观看 | 欧美黑人巨大最猛性xxxxx | 国产在线一二三区 | 国产日产欧美a级毛片 | 草久久久久 | 日韩欧美自拍 | 台湾一级特黄精品大片 | 国产美女一级毛片 | 久久精品国产99国产 |